In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods

A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro vivo plum genotypes ‘Valerija’, ‘?a?anska Lepotica’ ‘Valjevka’. In tested at day 0 (fresh dry pollen) after 3, 6, 9 12 months four (4, ?20, ?80 ?196 °C), distinct temperatures. germination fluorescein diacetate (FDA) staining methods were used test viability, while aniline blue for observing tube growth. Fresh ranged from 42.35 63.79% (‘Valjevka’ Lepotica’, respectively) 54.58 62.15%, respectively). With 4 °C, decreased over period, with lowest value storage. Pollen other (?20, °C) higher than 30% by end months. Pollination using stored °C showed that growth mostly ended lower part style. temperatures, similar, ranging between 50 100% pistils tubes penetrated into nucellus ovule genotype Lepotica’. The results these findings will have implications breeding conservation.